Journal:

Article Title: Diverse and Specific Gene Expression Responses to Stresses in Cultured Human Cells D⃞

doi: 10.1091/mbc.E03-11-0799

Figure Lengend Snippet: Conditions and cell lines used for microarray experiments

Article Snippet: Cell Culture Conditions Culture conditions were as follows: HeLa S3 cervical carcinoma cells ( Puck et al ., 1956 ) were grown in DMEM supplemented with 10% fetal bovine serum, 100 U of penicillin-streptomycin, and 1× nonessential amino acids at 37°C with 5% CO 2 ; normal human diploid lung fibroblasts (American Type Culture Collection, Manassas, VA) were grown under the same conditions as HeLa cells but with 20% serum; and K-562 cells ( Lozzio and Lozzio, 1975 ) were grown in spinner flasks at 37°C in Iscove's modified Dulbecco's medium supplemented with 10% fetal bovine serum and 100 U/ml penicillin-streptomycin.

Techniques: Microarray

Journal:

Article Title: Diverse and Specific Gene Expression Responses to Stresses in Cultured Human Cells D⃞

doi: 10.1091/mbc.E03-11-0799

Figure Lengend Snippet: Cell line-dependent gene expression patterns. Data for cell line specific clusters are shown with the same color scheme as in Figure 1. Clusters of genes whose expression was repressed by stress in fibroblasts (A), induced by stress in HeLa cells (B), or induced by redox stress in fibroblasts (C) were produced using the two-step clustering method described in MATERIALS AND METHODS. (D) We extracted and clustered the data for 1134 clones found to be cell cycle regulated by (Whitfield et al., 2002 ). The genes shown are the core of the resulting cluster and contain the most strongly cell cycle-regulated genes. (E) Data for all of the genes encoding ribosomal proteins present on the arrays were extracted and the genes were ordered by hierarchical clustering.

Article Snippet: Cell Culture Conditions Culture conditions were as follows: HeLa S3 cervical carcinoma cells ( Puck et al ., 1956 ) were grown in DMEM supplemented with 10% fetal bovine serum, 100 U of penicillin-streptomycin, and 1× nonessential amino acids at 37°C with 5% CO 2 ; normal human diploid lung fibroblasts (American Type Culture Collection, Manassas, VA) were grown under the same conditions as HeLa cells but with 20% serum; and K-562 cells ( Lozzio and Lozzio, 1975 ) were grown in spinner flasks at 37°C in Iscove's modified Dulbecco's medium supplemented with 10% fetal bovine serum and 100 U/ml penicillin-streptomycin.

Techniques: Gene Expression, Expressing, Produced, Clone Assay

Journal: Scientific Reports

Article Title: Extracellular vesicles from Paracoccidioides pathogenic species transport polysaccharide and expose ligands for DC-SIGN receptors

doi: 10.1038/srep14213

Figure Lengend Snippet: Data were normalized and the scale is indicated below the figures. ( a ) Unsupervised hierarchical clustering was performed for individual technical replicates. ( b ) Competitive inhibition of EV interactions with lectin microarray with mannose (Man) and N -acetylglucosamine (GlcNAc). ( c ) Lectin microarray of PNGase F-treated EVs. Significance (* = p ≤ 0.05) was determined by the ANOVA test.

Article Snippet: Probes (neoglyconjugates, glycoproteins and lectins) were printed on Nexterion® Slide H (Schott, Mainz, Germany) using a SciFlexArrayer S3 piezoelectric microarray printer (Scienion, Berlin, Germany) under constant 62% (+/−2%) humidity at 20 °C essentially as previously described .

Techniques: Inhibition, Microarray

Journal: ACS Omega

Article Title: Differential Glycosylation Expression in Injured Rat Spinal Cord Treated with Immunosuppressive Drug Cyclosporin-A

doi: 10.1021/acsomega.8b02524

Figure Lengend Snippet: Intensity of primary astrocyte and Neu7 cell protein extracts binding to lectins on microarray. Bar chart representing the differences in binding of fluorescently labeled protein extracts to printed lectins on a microarray surface where Ast_Lys is the total lysate from primary astrocytes, Ast_Cyt is the cytosolic (hydrophilic) protein-enriched extract from primary astrocytes, Ast_Mem is the membrane (hydrophobic) protein-enriched extract from primary astrocytes, Neu7_Lys is the total lysate from Neu7 cells, Neu7_Cyt is the cytosolic (hydrophilic) protein-enriched extract from Neu7 cells, and Neu7_Mem is the membrane (hydrophobic) protein-enriched extract from Neu7 cells. Graph represents the mean of three replicate experiments (except for duplicate experiments for Neu7_Mem binding to MAL-II), with each experiment the median of six individual replicates. Error bars represent ±1 standard deviation of the mean of the three experiments.

Article Snippet: The lectins were printed at approximately 1 nL per feature on Nexterion Slide H microarray slides using a sciFLEXARRAYER S3 piezoelectric printer (Scienion AG, Berlin, Germany) as previously described.

Techniques: Binding Assay, Microarray, Labeling, Standard Deviation